Generator

Part:BBa_K152005:Design

Designed by: David Johnston Monje   Group: iGEM08_Guelph   (2008-10-30)


Promoterless Synthetic Operon of CrtE,B,I,Y and GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1941
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1478
    Illegal NgoMIV site found at 1608
    Illegal AgeI site found at 732
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5199


Design Notes

The synthetic operon needed a transcriptional termination signal, as well as GFP to visualize complete transcript production, so BBa_E0240 was used. No more genes can be added to this operon with this GFP on the end.


Source

This operon is made from biobricks BBa_K152001 - BBa_K152003, the biobrick BBa_K118005 which was sent to us by iGEM Edinburg, and BBa_E0240.

References